Additional Cell Culture Reagents and Supplements
STEMCELL Technologies StemSpan™ Myeloid Expansion Supplement II (100X), Size: 1 mL
StemSpan™ Myeloid Expansion Supplement II (100X) contains a combination of recombinant human cytokines formulated to selectively promote the expansion and differentiation of human myeloid progenitor cells, generating large numbers of CD14+ monocytes from CD34+ cells isolated from human cord blood (CB) samples.StemSpan™ Myeloid Expansion Supplement II (100X) is intended for use in combination with any of the following StemSpan™ media:• StemSpan™ SFEM (Catalog #09600)• StemSpan™ SFEM II (Catalog #09605)• StemSpan™-XF (Catalog #100-0073)• StemSpan™-AOF (Catalog #100-0130)When added to serum-free medium, StemSpan™ Myeloid Expansion Supplement II typically promotes the production of hundreds of CD14+ monocytes per input CD34+ cell in 14-day liquid cultures initiated with CD34+ human CB cells. See data tab for more details.
Quality Biological Inc RPMI-1640 Medium without L-Glutamine, 500 mL
Suitable for a wide range of anchorage independent cells. ¿ Commonly supplemented with 10% Fetal Bovine Serum (FBS). ¿ With 2.0 g/L Sodium Bicarbonate ¿ Without HEPES buffer ¿ Applications: fusion protocols and growth of hybrid cells, culture of normal human leukocytes in monolayer and suspension, and support of HeLa, Jurkat, MCF-7, PC12, PBMC, astrocytes, and carcinoma growth (to name a few). ¿ RPMI 1640 Medium is unique from other media because it contains the reducing agent glutathione and high concentrations of vitamins. ¿ Storage Conditions: 2¿C to 8¿C¿ Shipping Condition: ambient temperature ¿ Sterile Filtered ¿ pH: 7.0 ¿ 0.2 ¿ Osmolality: 300 ¿ 5% mOsM/Kg H2O ¿ Product and/or packaging contains recycled/renewable content ¿ Product and/or packaging is recyclable or reduces waste ¿ Product and/or packaging is safer to human and environmental health ¿ Max Shelf Life: 24 months
Sigma Aldrich Fine Chemicals Biosciences Hanks' Balanced Salt solution HBSS Modified, with phenol red, without calcium, without magnesium, liquid | 1000ML
Hanks' Balanced Salt solution HBSS Modified, with phenol red, without calcium, without magnesium, liquid | 1000ML
STEMCELL Technologies STEMdiff™ Sensory Neuron Differentiation Kit, Size: 1 Kit
Generate functional, human-specific sensory neurons in a dish with this easy-to-use culture system. After obtaining pluripotent stem cell (PSC)-derived neural crest cells (NCCs) with STEMdiff™ Neural Crest Differentiation Kit (https://www.stemcell.com/products/stemdiff-neural-crest-differentiation-kit.html), use the serum-free STEMdiff™ Sensory Neuron Differentiation Kit to generate sensory neuron precursors. STEMdiff™ Sensory Neuron Maturation Kit can then be used to maintain and mature the sensory neuron precursors. The resulting cells are BRN3A-positive and more than 70% TUJ1-positive. With the physiological glucose and osmolarity conditions provided by BrainPhys™ (https://www.stemcell.com/products/brainphys-neuronal-medium.html) as the basal medium, the neurons exhibit activity in response to sensory ligands and changes in temperature. The generated population of functional human sensory neurons can be used for drug discovery and pain research applications.
Sigma Aldrich Fine Chemicals Biosciences Potassium chloride | 7447-40-7 | MFCD00011360 | 250 g
Potassium chloride | Purity: 99% | Mol Wt: 74.55 | 7447-40-7 | MFCD00011360 | 250 g
ATHENA ES Augmedium™, Powder 100
Augmedium™ is a medium additive which conditions cells prior to induction during a recombinant protein expression protocol. This pre-induction conditioner increases the level of chaperone proteins which can improve the fraction of product that accumulates as soluble protein. Augmedium™ is specifically intended for use with strains in which the target protein accumulates as an inclusion body or as an insoluble aggregate. Augmedium™ is supplied as a powder for preparation of 50x concentrated stock solutions. It is available in 100mL and 500mL amounts.
Sigma Aldrich Fine Chemicals Biosciences Poly-L-lysine hydrobromide mol wt 70,000-150,000 by viscosity | 25988-63-0 | MFCD00237230 | 500mg
Poly-L-lysine hydrobromide mol wt 70,000-150,000 by viscosity | 627.257 | 25988-63-0 | MFCD00237230 | 500mg
ILEX LIFE SCIENCES LLC PAN Biotech EBSS Earles Balanced Salts Solution with Ca and Mg and phenol red without NaHCO3 500 ML 1/EA
PAN-Biotech Earle s Balanced Salts Solution (EBSS) w Ca and Mg w/o NaHCO3 500 ml INTRODUCTION Earle s Balanced Salts Solution (EBSS) with calcium and magnesium without sodium bicarbonate APPLICATION The main purpose of buffered salt solutions is the maintenance of pH and osmolarity in cell culture applications as well as providing essential inorganic ions These solutions are usually used for washing tissues and cells Often they are combined with other agents in cell and tissue treatment BICARBONATE without Sodium Bicarbonate CALCIUM/MAGNESIUM with Ca and Mg CONCENTRATION 1x LIQUID/POWDER liquid PHENOL RED with Phenol Red STERILE Yes STORAGE TEMP 2 C - RT MANUFACTURER PAN-Biotech GmbH COUNTRY OF ORIGIN Germany USAGE This product is intended for Laboratory Research Use Only Not for use in diagnostic or therapeutic procedures
Mercodia Inc Animal Insulin Control(10-1221-01)Kit format: 3 x 0.5 mLMeasurement range: low, medium, high.
Animal Insulin Control(10-1221-01)Kit format: 3 x 0.5 mLMeasurement range: low, medium, high.Mercodia Animal Insulin Control (Low, Medium, High) is designed to be used as a three-level control for Mercodia Porcine, Bovine, Ovine, Canine and Equine Insulin ELISA kits.
Rainbow Scientific BUFFERED HYPOTONIC SOL.
It has been found to achieve good metaphase swelling (spreading) from a wide range of sample types and significantly increases the number of useful metaphases in leukaemic bone marrow cultures when compared to the standard 0.075M KCL hypotonic. It contains 0.4% KCL with HEPES.
Alkali Scientific CellPro™ HBSS with calcium and magnesium, 500ml
Balanced salt solution for cell culture applications (liquid form). All formulations incorporate a rigorous specification and approval process. Products with the LS and FS extension are supplied in convenient HyQPAK six-packs. Buffer cell culture growth with CellPro™ 1X Balanced Salt Solutions, available in lot sizes that facilitate scale-up and manufacturing.
Quality Biological Inc DPBS 1X with Calcium and Magnesium, 500 mL
DPBS is a balanced salt solution (BSS) ¿ Used for the handling and culturing of mammalian cells. ¿ Calcium and magnesium facilitate cell binding and clumping. ¿ DPBS without these ions can be used to wash and rinse suspended cells. ¿ Without Phenol red ¿ Applications: DPBS serves as an irrigating, transporting or diluting fluid for mammalian cells, while maintaining cell tonicity and viability for a limited period of time. ¿ Used as a diluent of fresh red blood cells to study the trapping of red blood cell aggregate ¿ Wash the wells of microtiter plates ¿ Wash cells during preparation and serial transfer. ¿ Storage Conditions: 2¿C¿8¿C ¿ Shipping condition: ambient temperature ¿ Sterile Filtered ¿ Product and/or packaging contains recycled/renewable content ¿ Product and/or packaging is recyclable or reduces waste ¿ Product and/or packaging is safer to human and environmental health ¿ ¿ Max Shelf Life: 24 months
InVitria ITSE+A Animal-Free - 10mLx10
InVitria’s ITS, ITSE, and ITSE+A Animal-Free are recombinant, blood-free cell culture media supplements used to optimize cell growth. For optimal proliferation and performance, cells cultured must be supplied with a critical proteins and components:Insulin: promotes uptake of glucose and amino acids, facilitating cellular signalingTransferrin: delivers intracellular iron via the transferrin receptor, drives cellular respiration, chelates free iron, and reduces generation of cytotoxic free radicals and peroxideSelenium: required for the activity of antioxidant enzymes such as glutathione peroxidase and thioredoxin reductaseEthanolamine: phospholipid precursor that improves the performance of cells in serum-free media and is required for the growth of certain cell typesAlbumin: binds, transports, and delivers ions, fatty acids, vitamins, hormones, small-molecule drugs, and toxins; regulates serum pH and osmotic pressure; and provides antioxidant activityMade in the USA.
Zymo Research Corporation Overexpression Broth (OB), 500 ml
Uniquely formulated Overexpression Broth™ (OB™) that is used in conjunction with our Expansion Broth™ (EB™) for consistent high level recombinant protein expression.